Oltipraz treatment in experimental schistosomiasis mansoni: VII effect of high dose oral therapy on alkaline phosphatase level in livers of infected mice

Biochemical estimation of alkaline phosphatase [AP] in livers of mice experimentally infected with the Egyptian strain of S. mansoni before and after oltipraz treatment was carried out. The drug was given in a single oral dose of 500 mg/kg body weight. The results revealed that the drug led to gradual reduction in the AP mean level after 20 and 30 days from treatment. This was then followed by rise in the enzyme mean level, after 60 days. The drug also caused significant reduction in the AP mean level in livers of the drug control group of mice after 5 and 20 days from oltipraz administration in comparison to normal uninfected controls. These findings might be attributed to a hepatocyto-toxic effect of the drug

prophylactic and curative effect of oltipraz in experimental schistosomiasis mansoni in mice

Effect of oltipraz on immature and mature forms of Schistosoma mansoni in mice experimentally infected with the Egyptian strain of the worms was assessed. The drug was administered to different groups of mice in a single oral dose of 750 mg/kg body weight 2 hours before infection, 7 days and 15 days after infection and in a single oral dose of 500 mg/kg body weight 30 days and 45 days after infection. Oltipraz was found to be active in reducing the number of worms significantly only when administered in a higher dosage 2 hours before infection and 7 days after infection. Thereafter, the parasites were refractory to the drug action until they have reached sexual maturity 45 days post-infection

Effect of oltipraz therapy on the total collagen content in livers of hamsters infected with schistosoma mansoni

Estimation of the total collagen content in livers of hamsters experimentally infected with the Egyptian strain of S. mansoni before and after oltipraz therapy was carried out. The drug was given in a single oral dose of 500 mg/kg body weight. The results obtained revealed that S. mansoni infection caused significant increase in the total collagen content of liver after 60, 70 and 80 days from infection in comparison to normal controls. Administration of oltipraz at an early stage of the disease [7 weeks after infection] led to significant reduction in the total collagen content after 11, 21 and 31 days from therapy in comparison to the respective infected untreated controls. The results obtained were discussed with those of others at the local and the international levels

Oltipraz treatment in experimental schistosomiasis mansoni: I morphological changes among S mansoni worms induced by high dose oral therapy in mice

Worms recovered from Oltiparz treated group of mice [500 mg/kg. body weight] show, stunted ness, absent or poorly developed tuberculations, destroyed or poorly developed suckers, absence or severe atrophy of testes, complete absence or severe atrophy of ovaries, small or empty vitelline follicles and slightly or not pigmented intestines as compared with controls; the female worms are more affected than males. The morphological changes are apparent after 5 days, 7 days, 20 days and 30 days from drug administration, but are reversible in some surviving worms especially male worms after 60 days from therapy. The previous findings indicate that the drug causes severe degenerative changes in the structure of both S. mansoni male and female worms and that resistance to the drug develops among the Egyptian strain of S. mansoni worms with prolongation the period of worms exposure to the drug. The present results are discussed with those of others with some recommendations

Oltipraz treatment in experimental schistosomiasis mansoni II effect of high dose oral therapy on worm load and process of copulation in infected mice

A single oral dose of 500 mg/kg body weight of Oltipraz was given to mice experimentally infected with the Egyptian strain of S. mansoni. The drug caused significant reduction in the number of worms and marked reduction in the percentage of paired worms in comparison to the infected untreated controls. This reduction in number was apparent after 5 days, 7 days and 2 days from Oltipraz administration, then the number gradually increased with elapsed time till the 60th day after therapy [the end of the work]. The previous findings might indicate development of resistance to Oltipraz among the Egyptian strain of S. mansoni worms with prolongation the period of worms exposure to the drug

Oltipraz treatment in experimental schistosomiasis mansoni: III effect of high dose oral therapy on hepatic shift of worms and tissue bould ova pattern in infected mice

Oltipraz was given in a single oral dose of 500 mg/kg body weight to mice experimentally infected with the Egyptian strain of S. mansoni. The drug caused marked hepatic shift of the worms in comparison to the infected untreated controls. Hepatic shift of worms was apparent till the 30th day from Oltipraz administration, while pronounced back shift to mesenteric veins by the surviving parasites occurred 60 days post- treatment. The drug also caused death of all bilharzial eggs located inside tissues of the small intestines of infected mice and complete eradication of fecal eggs after the 5th, 7th, 20th, 30th and 60th days from therapy. These findings need the histopathological investigations

Oltipraz treatment in experimental schistosomiasis mansoni: VI effect of high dose oral therapy on alkaline phosphatase level in the intestines of infected mice

Extraction of alkaline phosphatase [AP] from the tissues of small intestines of mice experimentally infected with the Egyptian strain of S. mansoni before Oltipraz treatment was carried out. The drug was given in a single oral dose of 500 mg/kg body weight. The results obtained revealed that the drug led to significant reduction in the AP mean level after 30 and 60 days from therapy in comparison to the infected untreated controls. Oltipraz also caused significant reduction in the intestinal AP mean level of the drug control group of mice after 5 and 20 days from drug administration in comparison to normal controls, which might be attributed to the toxic effect of the drug on the intestinal cells

Oltipraz treatment in experimental schistosomiasis mansoni: IV histopathological changes in the intestines of infected mice induced by high dose oral therapy

Marked improvement of the pathological lesions in the small intestines of mice experimentally infected with the Egyptian strain of S. mansoni was detected after Oltipraz therapy. The drug was given in a single oral dose of 500 mg/kg body weight. The improvement of the intestinal lesion was apparent after 20, 30 and 60 days from treatment in comparison to the infected untreated controls. These findings were the basis of the present study, which still need investigations of the histopathological changes in livers infected before and after Oltipraz therapy

Oltipraz treatment in experimental schistosomiasis mansoni: V histopathological changes in the livers of infected mice induced by high dose oral therapy

Histopathological changes in the livers of mice infected with the Egyptian strain of S. mansoni before and after Oltipraz therapy were studied. The drug was given in a single oral dose of 500 mg/kg body weight. The results obtained revealed marked improvement of the pathological lesions after 20 and 30 days from Oltipraz therapy in comparison to the infected untreated controls. That improvement was followed by a marked increase of hepatic lesions after 60 days from treatment, in addition to new cellular granulomata surrounding fresh bilharzial ova. The drug was severely toxic to the liver as it caused severe liver cell injury, parenchymatous inflammation, fatty changes and areas of diffuse necrosis in livers of the drug control group of mice. The results were discussed in the light of the previous and present findings detected by the authors, with some recommendations

Some histopathological changes in immuno-suppressed mice infected with hymenolepis nana var fraterna

Immune-suppression in Swiss Albino mice infected with H. nana and induced by administration of high and low doses of hydrocortisone acetate caused superinfection of the gut with H. fraterna worms and cysticercoids. There was also dissemination of cysticercoids to the liver with marked cellular infiltration, necrosis and destruction of the liver parenchyma. The pathogenesis caused by the disease whether in the gut or the liver was much more aggravated in the group of mice injected with the high dose of the drug. The control group of mice showed only few number of normal worms and cysticercoids in the gut without any extra-intestinal spread of cysticercoids to other organs. The results are discussed with those of others at the international level